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KMID : 0861020130280040071
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Korea Journal of Herbology
2013 Volume.28 No. 4 p.71 ~ p.76
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Comparative Study on Anti-inflammatory Effects and Compound Contents of Multi-herbal Extracts HT008 produced by Different Manufacturing Methods
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이동헌:Lee Dong-hun
송정빈:Song Jung-Bin/송미경:Song Mi-kyoung/:Bhakta Prasad Gaire/최호영:Choi Ho-Young/김호철:Kim Ho-Cheol/박주연:Park Ju-Yeon/김애홍:Ai hong Jin
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Abstract
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Objectives : The biological activities and compound contents of herbal medicine vary depending on manufacturing
processes. In this study, we compared anti-inflammatory effects and compound contents of three kinds of
multi-herbal extract HT008 produced by different manufacturing processes in order to determine chemical and
biological equivalence.
Methods : HT008 was produced by three different manufacturing methods: 1. Freeze dried extract of Eleutherococcus
senticosus, Scutellaria baicalensis and Angelica sinensis (HT008 FD), 2. Spray dried extract of E. senticosus and
S. baicalensis combined with reflux extract of A. sinensis (HT008 SD), 3. Spray dried extract of E. senticosus
and S. baicalensis combined with supercritial fluid extract of A. sinensis (HT008 SF). Anti-inflammatory effects
were evaluated using acetic acid induced pain model and λ-carageenan induced paw edema model. Compound
contents were evaluated by HPLC quantitative analysis of standard compounds of HT008, eleutheroside E,
baicalin, z-ligustilide.
Results : HT008 FD, HT008 SD and HT008 SF significantly decreased acetic acid induced pain index and λ -carrageenan induced paw edema volume compared with that of control group. There was no significant difference in efficacy among the HT008 FD, HT008 SD and HT008 SF. Standard compound contents of HT008 FD, HT008 SD and HT008 SF were quantified within the range of Korean pharmacopoeia or other research.
Conclusions : Three different manufacturing methods of multi-herbal extracts have been developed without
noticeable difference in the efficacy or compound contents. The results might be used to establish manufacturing
process and industrialization of herbal extracts.
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KEYWORD
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comparison of efficacy, compound contents, manufacturing method, , HT008, acetic acid, λ-carageenan, supercritical fluid extraction
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